Transduction of plasmid DNA in macrolide producing streptomycetes.
نویسندگان
چکیده
منابع مشابه
Transduction of plasmid DNA in macrolide producing streptomycetes.
Transduction was mediated by a 8-kb segment of bacteriophage FP43 DNAcloned into plasmid pIJ702: The resulting plasmid, pRHBlOl, was packaged in bacteriophage heads as linear concatemers and was transduced into about 80% of the Streptomyces species tested1}. We showed that the efficiency of transduction can vary markedly depending on the cell growth conditions, and that under optimum growth con...
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Plasmid pIJ7021) is a useful vector for the cloning and expression of streptomycete and mammalian genes in Streptomyces lividans.2~4) pIJ702 or its derivatives have broad host range, replicating in many Streptomyces species and in other actinomycetes (see ref 2~6 and refs therein). We have been interested in expanding the utility of pIJ702 by making it transducible. We cloned a segment of bacte...
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Actinomycetes were isolated from soybean rhizosphere soil collected as two field sites in Brazil. All the isolates were identified as Streptomyces species and were screened for streptomycin production and the presence of two genes, strA and strB1, known to be involved in streptomycin biosynthesis in Streptomyces griseus. Antibiotic resistance profiles were determined for 53 isolates from cultiv...
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A method for polyethylene glycol-induced protoplast transformation of glutamate-producing bacteria with plasmid DNA was established. Protoplasts were prepared from cells grown in the presence of penicillin by treatment with lysozyme in a hypertonic medium. The concentration of penicillin during growth affected the efficiency of formation, regeneration, and polyethylene glycol-induced DNA uptake...
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Pattee, P. A. (Iowa State University, Ames) and J. N. Baldwin. Transduction of resistance to some macrolide antibiotics in Staphylococcus aureus. J. Bacteriol. 84:1049-1055. 1962.-By use of phage 80 of the International Typing Series, propagated on appropriate strains of Staphylococcus aureus, two related markers controlling resistance to certain macrolide antibiotics (erythromycin, oleandomyci...
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ژورنال
عنوان ژورنال: The Journal of Antibiotics
سال: 1989
ISSN: 0021-8820,1881-1469
DOI: 10.7164/antibiotics.42.1725